Strategies for the purification of membrane proteins pdf

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This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins.

It explains the principles. Membrane proteins are classified based on the level of interaction with membrane lipid bilayers, with peripheral membrane proteins associating non-covalently with the membrane, and integral membrane proteins associating more strongly by means of hydrophobic interactions.

Generally speaking, peripheral membrane proteins can be purified by milder techniques than integral membrane proteins, whose extraction requires phospholipid bilayer disruption by detergents. Here, important criteria for strategies of membrane protein purification are addressed, with a focus on the initial stages of membrane protein solublilisation, where problems are most frequently encountered.

Protocols are outlined for the successful extraction of peripheral membrane proteins, solubilisation of integral membrane proteins, and detergent removal which is important not only for retaining native protein stability and biological func - tions, but also for the efficiency of later purification techniques.

Other Titles: Methods in Molecular Biology. Publisher: Springer. Type of material: Book Chapter. Availability: Full text available. Keywords: Protein solubilisation , Protein purification , Detergent , Integral membrane protein , Peripheral membrane protein.